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It can be examined at the cellular level in living preparations by differential interference contrast (DIC) microscopy, because it is transparent throughout its life cycle. A collagenous cuticle, secreted by the underlying epithelium, surrounds the worm on the outside and also lines the pharynx and rectum (see Cuticle). Changing synaptic specificities in the nervous system of : Differentiation of the DD motoneurons. The anatomical description of the whole animal has been completed at the electron microscopy level and its complete cell lineage, which is invariant between animals, has been established (Brenner, 1973; Byerly et al., 1976; Sulston et al., 1983; Wood, 1988a; Lewis and Fleming, 1995). Various tissues open to the outside through this cuticle (Intro FIG 3).
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The adult body plan is anatomically simple with about 1000 somatic cells.
is amenable to genetic crosses and produces a large number of progeny per adult.
It reproduces with a life cycle of about 3 days under optimal conditions.
The animal can be maintained in the laboratory where it is grown on agar plates or liquid cultures with as the food source.
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is a small, free-living soil nematode (roundworm) that lives in many parts of the world and survives by feeding on microbes, primarily bacteria (Intro FIG 1).
It is an important model system for biological research in many fields including genomics, cell biology, neuroscience and aging ( Electron microscopical reconstruction of the anterior sensory anatomy of the nematode (ed.
Among its many advantages for study are its short life cycle, compact genome, stereotypical development, ease of propagation and small size.
Self-fertilization of the hermaphrodite allows for homozygous worms to generate genetically identical progeny, and male mating facilitates the isolation and maintenance of mutant strains as well as moving mutations between strains. DIC image of an adult hermaphrodite, left lateral side. Dotted lines and numbers mark the level of each section shown in Intro FIG 2. Two cuticular inpockets form narrow openings at the lateral lips for the amphid sensilla (Intro FIG 4A and Intro TABLE 1). points to cytoplasmic material (tubule-associated material, TAM) within PDE (posterior deirid) neuron ending in the cuticle.